Although several adhesive resin-based sealers have recently been introduced, chemical surface analyses of adhesive interface have not yet been studied. In this study, Energy-dispersive x-ray spectroscopy (EDS) microanalysis, Fourier transform infrared (FT-IR) analysis and Scanning electron microscopy (SEM) observation were executed to investigate chemical characteristics of roots filled with a 4-META-containing resin-based MetaSEALTM (Hybrid Root SEAL; equivalent product to MetaSEALTM, marketed in Europe by Sun Medical, Japan).
Energy-dispersive x-ray spectroscopy (EDS) microanalysis, Fourier transform infrared (FT-IR) analysis and Scanning electron microscopy (SEM) observation were executed to investigate chemical characteristics of roots filled with Hybrid Root SEAL (HRS)
SEM revealed that resin tags and hybrid-like layer were formed in HRS/dentin interface, and resin-impregnated layer without interfacial gap-formation was observed in the HRS/GP interface.
EDS element analysis demonstrated different calcium, silicon, carbon and zinc contents in radicular dentin, hybrid-like layer, HRS, resin-impregnated layer and GP, respectively. EDS line-scan showed the amount of zinc rapidly decreased from GP towards to the resin-impregnated layer, but the amount of carbon gradually increased in resin-impregnated layer.
Interface between MetaSEAL TM and radicular dentinFig. 2 Line-scan in the vicinity of resin-dentin interface.Fig. 4 SEM micrograph of a cross-sectional view.Fig. 3 Superimposition of EDS element mapping.Interface between MetaSEAL TM and GPFig. 5 SEM micrograph of a cross-sectional view.Fig. 6 Line-scan in the vicinity of resin-GP interface.
FT-IR analysis showed that the absorption peaks of hybrid-like layer were similar to these of radicular dentin and HRS.
These results indicated that Hybrid Root SEAL could bond to radicular dentin and GP via the formation of hybrid layers.